Development of the method of clonal micropropagation of the stone fruit crops rootstock Gisela 6 (Prunus cerasus ‘Schattenmorelle’ x Prunus canescens Bois)

Gisela 6 is one of the semi-dwarf own-rooted rootstocks of stone fruit crops, which is used to create and develop modern intensive stone fruit orchards. Reproduction of the rootstock in large volumes using traditional methods is expensive and labor-intensive, which determines the use of the clonal micropropagation method, which will allow you to get a huge number of genetically homogeneous and virus-free plants in a short period of time. As a result of the studies, a protocol for clonal micropropagation of Gisela 6 was developed. It was found that the MS nutrient medium supplemented with growth regulators 6-BAP, GA3 and IBA at concentrations of 1 mg / l, 0.5 mg / l and 0.1 mg / l, respectively (on the 30th day, the multiplication factor was 6.5) is the most optimal medium for cultivation. The most effective medium for rooting is MS, supplemented with IBA at a concentration of 4.0 mg / l (on the 28th day, the efficiency of rhizogenesis reached 100%). The efficiency of adaptation to soil conditions of protected ground was 83.3%.

1. Aka-Kacar Y, Akpinar C, Agar A, Yalcin-Mendi Y, Serce S, Ortas I The effect of mycorrhiza in nutrient uptake and biomass of cherry rootstocks during acclimatization // Romanian Biotechnological Letters. 2010. Vol 15. №. P. 5246–5252.

2. Buyukdemirci H. The effects of medium ingredients on shoot propagation and rooting of cherry rootstocks in vitro // Acta Horticulturae. 2008. Vol 795. P. 419-422

3. Carolina A., Paula M., Pijut C., Michler H. Adventitious shoot regeneration and rooting of Prunus serotina in vitro cultures // Horticulture Science. 2006. Vol 41(1). P. 193-201.

4. Driver J.A., Kuniyuki A.H. In Vitro Propagation of Paradox Walnut Rootstock // Hort Science. 1984. Vol 19. P. 507-509.

5. Erbenova M, Paprstein F., Seldak J. In vitro propagation of dwarfed rootstocks for sweet cherry// Acta Horticulture. 2001. Vol. 560. P. 477-480.

6. Hossini A.D. Effects of media cultures and plant growth regulators in micro propagation of Gisela 6 rootstock // Annals of Biological Research. 2010. №. 2. P. 135-141.

7. Kuznetsova A., Drigina F., Fedorenko A., Maslova M. Identification of new resistant rootstocks for large-podded stone crops in the conditions of Southern Russia// Fruit growing and viticulture of South Russia. 2020. 64(4). 128-142. http://doi.org/10.30679/2219-53352020-4-64-5

8. Moghaddam R.N. et all. In vitro culture of gisela 6 semi-dwarf rootstock // Journal of Biological and Environmental Sciences. 2013. Vol. 7(20). P. 57-64.

9. Mourenets L.Y. and. Dolgov S.V. Adventitious shoot regeneration from leaf explants of two apricot rootstocks // Acta Hortic. 2016. Vol. 1139. P. 501139. DOI 10.17660/ActaHortic.

10. Murashige T., Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures // Physiol Plant. 1962. Vol. 15(3). P.473-497.

11. Orlova L, Scheglov S., Kuznetsova A. Fruit growing and viticulture of South Russia // Fruit growing and viticulture of South Russia. Vol. 58. №4. P. 46-57 (2019). http://doi.org/10.30679/2219-5335-2019-4-58-46-57.

12. Quoirin M., Lepoivre P. Improved media for in vitro culture of Prunus sp. // Acta Hortic. 1977. Vol 78. P. 437-442

13. Roy A., Fayad A., Barthe R.H., Brlansky R.H. A multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of multiple viruses in citrus trees // Journal of Virological Methods. 2005. Vol. 129(1). P. 47-55

14. Ruzic D., Saric M., Cerovic R., Culafic L. Plant Cell, Tissue and Organ Culture // Plant Cell, Tissue and Organ Culture. 2000. 63(1). Р. 9-14.

15. Sánchez-Navarro J.A., Aparicio F., Herranz M.C. et al. Simultaneous detection and identification of eight stone fruit viruses by one-step RT-PCR // Eur J Plant Pathol. 2005. Vol. 111. P. 77–84. https://doi.org/10.1007/s10658-004-1422-y

16. Song G.Q., Sink K.C. Transformation of Montmorency sour cherry (Prunus cerasus L.) and Gisela 6 (P. cerasus× P. canescens) cherry rootstock mediated by Agrobacterium tumefaciens // Plant Cell Reports. 2006. Vol. 25. P. 117-123.

17. Tang H., Ren Z., Reustle G., and Krczal G. Plant regeneration from leaves of sweet and sour cherry cultivars // Sci. Hortic. 2002. Vol. 93. P. 235–244

18. Vinter M. et al. Micropropagation of rootstocks of stone fruit cultures in vitro // BIO Web of Conferences. EDP Sciences, 2020. Т. 25. Р. 05001