Creation and maintanance of in vitro collections of ornamental cultures from the Lomonosov MSU Botanical Garden’s funds
https://doi.org/10.36305/2712-7788-2020-3-156-55-64
Abstract
The article contains the data on creating and maintaining the collections in vitro of some ornamental plants – lilac, roses, tree peonies. The analysis of morphogenetic processes during microclonal propagation of lilac cultivars demonstrates well-defined correlation between susceptibility of this culture to mineral mixture of nutrient medium and plant genotype. Rose cultivars from different garden groups keep high growth capacity during 12 subculturing cycles on Murashige Skoog medium (MS) supplemented with 0.5 mg/l BAP. For all culturing tree-peonies cultivars Woody Plant Medium (WPM) with double content of Ca2+ ions and total exception of Cl- ions turns out to be more suitable. The opportunity of 10 lilac cultivars and 4 rose cultivars preservation in slow growth cultures was appreciated. The correlation between long-term cultivation in low temperature and genotype of ornamental plants cultivars was shown. Thus, 18 ºС turns out to be suitable for creation of slow-growth collection of all studied rose cultivars, and 14 ºС – only for 'Nina Weibull'. Maintaining of lilac microshoots at 10 ºС during 5 months practically did not act negatively on their viability.
Supporting agencies: The work was carried out within the framework of the state research program " Study of morphogenesis patterns and formation of productivity elements under the influence of environmental factors; development of principles of morpho-physiological classification of plants "(AAAA16-116021660105-3) and supported by the "Project to increase the competitiveness of leading Russian universities among the world's leading research centers: 5-top 100" (Sechenov University).
About the Authors
O. A. Churikova
Московский Государственный Университет им. М.В. Ломоносова
Russian Federation
Russian Federation
A. A. Krinitsina
Московский Государственный Университет им. М.В. Ломоносова; Первый Московский государственный медицинский университет им. И.М.Сеченова
Russian Federation
Russian Federation
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Review
For citations:
Churikova O.A., Krinitsina A.A. Creation and maintanance of in vitro collections of ornamental cultures from the Lomonosov MSU Botanical Garden’s funds. Plant Biology and Horticulture: theory, innovation. 2020;(156):55-64. (In Russ.) https://doi.org/10.36305/2712-7788-2020-3-156-55-64
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