The effect of pre-harvest treatment with an herbicide on the accumulation dynamics and composition of essential oil of Elsholtzia ciliate (Thunb.) Hyl.

In the field experiment, the effect of pre-harvest treatment with an aqueous solution of various concentrations with the herbicide "Alistair grand" was studied, which includes diflufenicane - an inhibitor of phytoindesaturase (HRAC: F1 group) on the dynamics of accumulation and composition of the essential oil Elsholtzia ciliata (Thunb.) Hyl.  The composition of the oil was determined by the HZHK-MS method. The data obtained showed that already on the seventh day after treatment, the natural hormonal balance of the course of biosynthetic processes is disturbed. At a concentration of the drug solution of 0.05 g/l, the intensity of oil biosynthesis in plants decreases, on the 14th day it drops by almost a third compared to the control. At a solution concentration of 0.005 and 0.0005 g/l on the seventh day, under the influence of the drug, the energy of EO synthesis increases and its content in plants increases by 10% compared to control. However, even at these concentrations, the drug has a strong inhibitory effect on the processes of EO biosynthesis. On the fourteenth day of post processing, the oil content is reduced by 10-20%. Changes in the multi-enzymatic complex can also be traced in the redox equilibrium of the system, which affected the change in the biosynthesis of the main components of the EO. At a solution concentration of 0.005 and 0.0005 g/l on the seventh day after treatment, recovery processes are activated and the content of ketone elscholzium oil increases. 

1. Zhiqin Guo, Zizhen Liu, Xiaohong Wang, Weirui Liu, Rui Jiang, Ruiyang Cheng and Gaimei She. Elsholtzia: phytochemistry and biological activities // Chemistry Central Journal 2012. No. 6. P.147.

2. Komarov V.L. Borisova A.G., Vasilchenko E.V. et al. Flora SSSR. Family Labiatae. Vol. 20. M.; L.: Izd-vo AN SSSR, 1954.

3. Brandorf A.Z., Shestakova A.I., Galitskaya D.V., Larkina E.O., Sabitova L.Sh. Efficiency of the use of comb shandra (Elsholtzia cristata) in feeding bee colonies // In the collection: Prospects for the development of beekeeping in the conditions of industrialization of the agroindustrialization of the agro-industrial complex. Collection of articles on the materials of the International Scientific and Practical Conference. 2020. Р. 120.

4. Xu L., Bai J., Xing X., Cui H., Peng J., Zheng X.Сюй Л. Soil biological characteristic, nutrient contents and stoichiometry as affected by different types of remediation in a smelterimpacted soil. // Chemistry and Ecology. 2020. Vol. 36. № 5. P. 419-433

5. Li S.-Z., Zhu X.-K., Wu L.-H., Luo Y.-M.Ли С. З., Чжу Х.К., УЛ.Х., LUO Y.M., Zinc, iron, and copper isotopic fractionation in Elsholtzia splendens Nakai: a study of elemental uptake and (re)translocation mechanisms // Journal of Asian Earth Sciences. 2020. Vol. 192. P. 104227.

6. Shain S.S., Kurapov P.B., Malankina E.L. et al. Hormonal regulation of bioproductivity in ontogenesis of essential oil plants: peppermint, snakehead Moldavian, monarda dvuchataya / V sb. Biotechnology pod red. V.S. Shevelukha. 2000. № 1. Р. 176-198.

7. Sushkova L.O., Dmitrieva V.L., Dmitriev L.B. Influence of plant treatment with herbicides on the character of biosynthesis of essential oil Mentha piperita L. variety Yantarnaya // Butler messages. 2013. T. 34. № 34. P. 149–151.

8. Dmitrieva V.L., Sushkova L.O., Dmitriev L.B. Regulation of the ratio of components of essential oil Elsholtzia ciliata (Thunb.) Hyl. In the collection TSA Reports. 2020. Р. 405-409.

9. Dmitrieva V.L., Sushkova L.O., Dmitriev L.B., Belopukhov S.L. Regulation of the ratio of components of essential oil Elsholtzia ciliata (Thunb.) Hyl. in the conditions of the nonchernozem zone of Russia // In the collection: Prospects of medicinal plant science. Materials of the International Scientific Conference dedicated to the 100-th anniversary of the birth of Professor Alexey Ivanovich Schroeter. 2018. Р. 87-92.

10. Baybekov R.F., Dmitrieva V.L., Belopukhov S.L., Dmitriev L.B., Sushkova L.O. Influence of the herbicide diflufenicane on the chemical composition of the essential oil Elsholtzia ciliata (Thunb.) Hyl. Issues of biological, medical and pharmaceutical chemistry. 2020. Vol. 23. No. 4. Р. 3-9.