Experimental modeling of biofilm: structure, destruction and imaging
Abstract
This work was aimed to study the cellular population of a biofilm formed on porous surface, as well as to investigate a method for its destruction using electrochemically activated solutions. The study was carried out on an experimental stand in the form of a water recirculation system with the possibility for infecting microflora to cultivate the biofilms. As a starting culture, a dried mixture of microorganisms ( Lactococcus lactis , Streptococcus thermophilus , Lactobacillus acidophilus , Lactobacillus helveticus , Propionibacterium freudenreichi i ssp. Shermani ) was applied. The biofilm formation was examined with electron scanning microscopy. Then, the approaches to destroy the bacterial biofilms were studied using electrochemically activated solutions. The analysis of microphotographs showed that electrochemically activated water allows damaging effect on bacterial biofilm. This work was supported by the Russian Science Foundation grant 17-76-20014.
About the Authors
M. A. Pogorelova
ФГБУН Институт теоретической и экспериментальной биофизики РАН
Russian Federation
Russian Federation
A. L. Kuznetsov
ФГБУН Институт теоретической и экспериментальной биофизики РАН
Russian Federation
Russian Federation
O. A. Suvorov
ФГБУН Институт теоретической и экспериментальной биофизики РАН
Russian Federation
Russian Federation
L. G. Ipatova
ФГБУН Институт теоретической и экспериментальной биофизики РАН
Russian Federation
Russian Federation
A. G. Pogorelov
ФГБУН Институт теоретической и экспериментальной биофизики РАН
Russian Federation
Russian Federation
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Review
For citations:
Pogorelova M.A., Kuznetsov A.L., Suvorov O.A., Ipatova L.G., Pogorelov A.G. Experimental modeling of biofilm: structure, destruction and imaging. Plant Biology and Horticulture: theory, innovation. 2017;(144 (2)):152-155. (In Russ.)
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