scbookБиология растений и садоводство: теория, инновацииPlant Biology and Horticulture: theory, innovation2712-7788Federal State Funded Institution of Science “The Labour Red Banner Order Nikitsky Botanical Gardens – National scientific Center of the RAS”scbook-177Research ArticleХРАНЕНИЕ И ПЕРЕРАБОТКА ПЛОДОВО-ЯГОДНОЙ И ОРЕХОПЛОДНОЙ ПРОДУКЦИИЭКСПЕРИМЕНТАЛЬНОЕ Моделирование биопленки: СТРУКТУРА, дезинтеграция и визуализацияExperimental modeling of biofilm: structure, destruction and imagingПогореловаМ. А.PogorelovaM. A.noemail@neicon.ruКузнецовА. Л.KuznetsovA. L.noemail@neicon.ruСуворовО. А.SuvorovO. A.noemail@neicon.ruИпатоваЛ. Г.IpatovaL. G.noemail@neicon.ruПогореловА. Г.PogorelovA. G.agpogorelov@rambler.ruФГБУН Институт теоретической и экспериментальной биофизики РАНRussian Federation2017231020190144 (2)152155Copyright © Погорелова М.А., Кузнецов А.Л., Суворов О.А., Ипатова Л.Г., Погорелов А.Г., 20192019Погорелова М.А., Кузнецов А.Л., Суворов О.А., Ипатова Л.Г., Погорелов А.Г.Pogorelova M.A., Kuznetsov A.L., Suvorov O.A., Ipatova L.G., Pogorelov A.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://scbook.elpub.ru/jour/article/view/177

Цель данной работы заключалась в том, чтобы исследовать популяцию клеток в биопленке, сформированной на пористой поверхности, а также изучить возможность ее удаления посредством элктрохимически активированного водного раствора. Исследование проводили на лабораторном стенде в виде рециркуляционной системы, заполненой средой культивирования с микрофлорой (Lactococcus lactis, Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus helveticus, Propionibacterium freudenreichii ssp. Shermani). Структуру сформированной биопленки изучали с помощью сканирующей электронной микроскопии. Анализ полученных микрофотографий показал, что католит, элктрохимически активированный водный раствор обеспечивает дезинтеграцию биопленки.

This work was aimed to study the cellular population of a biofilm formed on porous surface, as well as to investigate a method for its destruction using electrochemically activated solutions. The study was carried out on an experimental stand in the form of a water recirculation system with the possibility for infecting microflora to cultivate the biofilms. As a starting culture, a dried mixture of microorganisms ( Lactococcus lactis , Streptococcus thermophilus , Lactobacillus acidophilus , Lactobacillus helveticus , Propionibacterium freudenreichi i ssp. Shermani ) was applied. The biofilm formation was examined with electron scanning microscopy. Then, the approaches to destroy the bacterial biofilms were studied using electrochemically activated solutions. The analysis of microphotographs showed that electrochemically activated water allows damaging effect on bacterial biofilm. This work was supported by the Russian Science Foundation grant 17-76-20014.

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The authors declare that there are no conflicts of interest present.